SBI Microscopy Facility

Overview of Services


 

 

The Microscopy division of the School of Biochemistry and Immunology at TCD is integrating advances in Microscopy with Computer Imaging. The services of this facility are available to all researchers, both internal and external to the University. The instruments are designed for analysis of multiple dyes on fixed or live samples (e.g. multicolor immunofluorescence, photoactivation, colocalisation studies FRET & FRAP). It has been developed to facilitate research in the biological sciences through the use of state of the art imaging systems.

 

We have a Leica SP8 gated STED with 5 detectors and a white laser. It also has a tandem scanner that allows you to take 24 frames a second. We also have a standard Leica SP8 confocal with 3 detectors.

 

 We have a Olympus FV1000 scanning confocal for both fixed and live cell imaging in high resolution which also allows for building of 3D images for better spatial resolution and an Andor spinning disk confocal for high speed live imaging.

 

We have two systems for epifluorescence imaging, an Olympus BX51 upright microscope with four colour LED and an IX81 inverted microscope for long working distance.

 

We also have a Nikon Eclipse E400 polarising microscope. Along with the equipment we provide application support, including experimental setup, sample preparation advice and troubleshooting along with software analysis of the results using Imaris software from Bitplane.

 

We have a tender in for new super resolution confocal system which will be the first of its kind in Ireland and a new scanning confocal microscope. These new systems will greatly expand the ability of the facility to cover the research needs of all the schools in Trinity.

 

Leadership

 

Dr. Gavin McManus

Manager

01-8964326

mcmanug@tcd.ie

http://www.tcd.ie/Biochemistry/confocal

 

Location

Facility is located in B2.46 TBSI 

 

Publications using our facility

 

NLRP3 has a protective role in age-related macular degeneration through the induction of IL-18 by drusen components.

Nat Med. 2012 Apr 8.

 

Doyle SL, Campbell M, Ozaki E, Salomon RG, Mori A, Kenna PF, Farrar GJ, Kiang AS, Humphries MM, Lavelle EC, O'Neill LA, Hollyfield JG, Humphries P.

 

IFI16 is an innate immune sensor for intracellular DNA.

Nat. Immunol. 11, 997-1005. 2010

 

Unterholzner, L., Keating, S.E., Baran, M., Horan,K.A., Jensen, S.B., Sharma, S., Sirois, C.M., Jin, T., Latz, E., Xiao, T.S., Fitzgerald, K.A., Paludan, S.R. & Bowie, A.G..,

 

Microglia in the degenerating brain are capable of phagocytosis of beads and of apoptotic cells, but do not efficiently remove PrPSc, even upon LPS stimulation

 

Glia. 2010 December; 58(16): 2017–2030.

MARTINA M. HUGHES,1 ROBERT H. FIELD,1 V. HUGH PERRY,2 CAROL L. MURRAY,1

AND COLM CUNNINGHAM1

 

Photophysical and biological investigation of novel  luminescent Ru(II)-polypyridyl-1,8-naphthalimide Tröger's bases as cellular imaging agents


Chem. Commun., 2012, 48, 2588-2590

Robert B. P. Elmes ,  Marialuisa Erby ,  Sandra A. Bright ,  D. Clive Williams and Thorfinnur Gunnlaugsson

Contacts

Name Role Phone Email Location
Dr. Gavin McManus
Manager
 
01-8964326
 
mcmanug@tcd.ie